Discovery Metabolomics Subcore

The Discovery Metabolomics Sub Core of the NORC provides Affiliate Investigators with a wide range of advanced, broad-based targeted and global metabolomics assays for comprehensive and detailed information on metabolism at the cellular or organism level as it relates to nutrition, obesity and diabetes.

Targeted Metabolite Profiling

Multi-Pathway Assays

• Aqueous: >350 metabolites in >50 pathways
• Lipidomics: 1100 lipids from 13 classes

Focused Assays

• Tryptophan (20 metabolites)
• Acyl Carnitines (40)
• TCA cycle (13 metabolites)
• Bile Acids (55)
• Other specific pathways, Co-A’s, cardiolipins, etc.

Global Metabolite Profiling 

• Aqueous metabolites (~400 + 1000 unknowns) by LC-MS
• Organic, amino, fatty acids (~150) by GC-MS
• NMR analysis (intact tissue, isotope tracers)
• Lipidomics (600+ lipids) by LC-MS

Flux Analysis (NMR- and GC-MS-Based)

Statistical and Pathway Analysis

• Multi-analyte significance testing
• Multivariate statistical modeling
• Metabolic Pathway analysis